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Techniques and Resources

Computational mitochondrial biology

Picture
Picture

Laboratory assays of mitochondrial behavior

RNA/DNA purification and sequencing
Fluorometry
Permeabilized tissues & cells
DNA gel electrophoresis
Enzymatic activity assays
Histology
Respirometry
Automated liquid handling
Multiplex qRT-PCT
Digital PCR
Confocal microscopy
Electron microscopy
Tissue culture
EM Tomography
3D electron microscopy resolution imaging of mitochondrial networks.

Cell Rep (2019)

Time-lapse live cell imaging of a mouse embryonic fibroblast labeled with Mitotracker Green. The central round structure is the cell nucleus. Duration: approximately 3 minutes in real time.

Trends Neurosci (2015), 38(8):468-74 

Electron tomogram of mouse cardiomyocyte mitochondria exhibiting trans-mitochondrial cristae coordination.

Nat Commun (2015), 6:6259
Downloadable video

Snapping cellular protrusion from HEK293 cells, imaged by confocal microscopy. 

​Tubular membrane structures analogous to mitochondrial nanotunnels.
  • Home
  • Research
    • Mitochondrial Signaling & Aging
    • Stress Psychophysiology and Allostatic Load
    • Mitochondrial Disease & the Brain
    • Techniques and Resources >
      • Protocols
  • Publications
  • Members
    • Lab Pictures
    • Lab Alumni
  • DATA
  • Contact