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Laboratory Techniques

Computational mitochondrial biology

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Laboratory assays of mitochondrial biology

RNA/DNA purification and sequencing
Fluorometry
Permeabilized tissues & cells
DNA gel electrophoresis
Enzymatic activity assays
Histology
Respirometry
Automated liquid handling
Multiplex qRT-PCT
Digital PCR
Confocal microscopy
Electron microscopy
Tissue culture
EM Tomography
3D electron microscopy imaging of the mitochondrial networks in the soma of a pyramidal neuron from the dentate gyrus of a C57BL/6J mouse brain.

​Each mitochondrion is a different color.

Faitg et al. Cell Rep (2021)

3D electron microscopy imaging of the mitochondrial networks of a dendrite from the dentate gyrus of a C57BL/6J mouse brain.

Faitg et al. Cell Rep (2021)

Time-lapse live cell imaging of a human endothelial cells labeled with Mitotracker Green.

Picard, Trumpff, Burelle. Curr Opin Behav Sci (2020)

Time-lapse live cell imaging of a mouse embryonic fibroblast labeled with Mitotracker Green. The central round structure is the cell nucleus.

​Duration: approximately 3 minutes in real time.

Picard M. Trends Neurosci (2015) 

Electron tomogram of mouse cardiomyocyte mitochondria exhibiting trans-mitochondrial cristae coordination.

Nat Commun (2015), 6:6259
Downloadable video

Live cell imaging showing a snapping cellular protrusion from HEK293 cells, imaged by confocal microscopy. 

​Tubular membrane structures analogous to mitochondrial nanotunnels.
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  • Home
  • Research
    • MiSBIE
    • Laboratory techniques
  • Members
    • Lab Pictures
    • Lab Alumni
  • PUBLICATIONS
    • All Publications
    • Publications by Category >
      • Mitochondrial Diversity
      • Mitochondrial Diseases
      • Mind-Mitochondria
      • Aging
      • Biomarkers
      • Energetics of Health
  • RESOURCES
    • Presentation Slides
    • Fawcett 1966
  • Stories
  • Contact